Cell Technology/Fluoro MPO/500/FLMPO100,Cell Technology,,Cell Technology,celltechnology,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Cell Technology/Fluoro MPO/500/FLMPO100

产品编号：FLMPO100

市场价：¥8900.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>限制性内切酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$445.00

品牌： Cell Technology

公司：CellTechnology

公司分类：

商品介绍

Description
Additional Information
Readable Documents
Assay Principle
Reviews

Key Benefits

Can monitor multiple time points to follow kinetics.
One-step, no wash assay.
Adaptable for High Throughput format.
Highly Sensitive.
Applications – Fluorescent Plate Reader or Absorbance Plate Reader.

Additional information

Kit Size	500

Detection of MPO activity in neutrophils and macrophages.
Detection of PMN infiltration in tissue samples (inflammation and innate host defense mechanisms).
Acute and chronic inflammatory disorders due to oxidative tissue damage.
MPO activity in acute and chronic manifestations of cardiovascular disease.

myeloperoxidase_mpo

Figure 1. MPO standard curve was serially diluted in 1X Reaction Buffer. Reaction cocktail (RC) was prepared as described (without EPO inhibitor). Next 50uL of MPO standard and 50uL of RC was added to individual well of a 96 well black plates. The plate was incubated at room and temperature in the dark. Data collected Ex:530nm Em:590nm

Document Title

MPOProtocol

Fluoro MPO Datasheet

msds.fluoroMPO

Title	File	Link	Author(s)	Journal	Year; Edition:Pages
Use of a hanging-weight system for liver ischemic preconditioning in mice		http://ajpgi.physiology.org/cgi/content/abstract/294/6/G1431	Hart, Much, Kohler et.al	Am J Physiol Gastrointest Liver Physiol	294: G1431-G1440, 2008
Extracellular Adenosine Production by Ecto-5′-Nucleotidase Protects During Murine Hepatic Ischemic Preconditioning		http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFX-4T4HP30-3&_user=10&_coverDate=11%2F30%2F2008&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1245872620&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=0348e5d389c76f43fa0ca609f91a05f7	M. Hart, C. Much, I. Gorzolla, J. Schittenhelm, D. Kloor, G. Stahl, H. Eltzschig	Gastroenterology	Volume 135, Issue 5, Pages 1739-1750.e3
Inflammatory Biomarkers of Sulfur Mustard Analog 2-Chloroethyl Ethyl Sulfide–Induced Skin Injury in SKH-1 Hairless Mice		http://toxsci.oxfordjournals.org/cgi/content/abstract/108/1/194	Neera Tewari-Singh, Sumeet Rana, Mallikarjuna Gu, Arttatrana Pal, David J. Orlicky, Carl W. White and Rajesh Agarwal	Toxicological Sciences	2009 108(1):194-206

Reference

Olsen, R. L. & Little, c. (1983) Biochem. J. 209, 781-787.

Nauseef, W. M., and Malech, H. L. (1986) Blood, 67, 1504-1507.

Andrews, P. C., Parnes, C., and Krinsky, N. I. (1984) Arch. Biochem. Biophys., 228, 439-442.

Mark B. Hampton, Anthony J. Kettle, and Christine C. Winterbourn. Inside the Neutrophil Phagosome: Oxidants, Myeloperoxidase, and Bacterial Killing. Blood, Vol. 92 No. 9 (November 1), 1998: pp. 3007-3017.

Andrews, P.C., Parnes, C. & Krinsky, N.I. (1984) Comparison of myeloperoxidase and hemi-myeloperoxidase with respect to catalysis, regulation, and bacterial activity. Arch. Biochem. Biophys. 228, 439–442.

Mingjie Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina and Richard P. Haugland. A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases. Anal Biochem 253, 162 (1997).

J. G. Mohanty, Jonathan S. Jaffe, Edward S. Schulman and Donald G. Raible. A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative. J. Immunol Methods 202, 133 (1997).

Tatyana V. Votyakova and Ian J. Reynolds. Membrane Potential dependent and -independent production of reactive oxygen species by rat brain mitochondria. J Neurochem 79, 266 (2001).

Chun Song, Abu B. Al-Mehdi, and Aron B. Fisher. An immediate endothelial cell signaling response to lung ischemia. Am J Physiol Lung Cell Mol Physiol 281, L993 (2001).

Samantha C. Richer and W.C.L. Ford. A critical investigation of NADPH oxidase activity in human spermatozoa. Mol Hum Reprod 7, 237 (2001).

William G. Gutheil, Miglena E. Stefanova and Robert A. Nicholas. Fluorescent Coupled Enzyme Assays for -Alanine: Application to Penicillin-Binding Protein and Vancomycin Activity Assays. Anal Biochem 287, 196 (2000).

Dominik Peus, Remus A. Vasa, Astrid Beyerle, Alexander Meves, Carsten Krautmacher and Mark R. Pittelkow. UVB Activates ERK1/2 and p38 Signaling Pathways via Reactive Oxygen Species in Cultured Keratinocytes. J Invest Dermatol 112, 751 (1999).

Tatyana V.Votyakova ,Ian J.Reynolds. Detection of hydrogen peroxide with Amplex Red:interference by NADH and reduced glutathione auto-oxidation. Archives of Biochemistry and Biophysics, 431: 138-144 (2004)..

Elizabeth Forbes, Tosei Murase, Ming Yang, Klaus I. Matthaei,*James J. Lee, Nancy A. Lee, Paul S. Foster, and Simon P. Hogan. Immunopathogenesis of Experimental Ulcerative Colitis Is Mediated by Eosinophil Peroxidase. The Journal of Immunology, 2004, 172:5664–5675.

Part#	Reagent	Temperature
Part# 4007	Detection Reagent, 1 Vial	-20C
Part# 3002	10X Assay Buffer, 60mL	2-8C
Part# 3012	Hydrogen Peroxide, 1000µL of a Stabilized 3% Solution	2-8C
Part# 6015	Myeloperoxidase, 1 Vial at 30Units/mL	2-8C

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Cell Signaling Technology (CST) 是一家由科学家创立的私营家族公司，致力于提供全球最高品质的创新研究和诊断产品，加速生物学认知以及实现个体化医疗。CST坚持自主生产和严格验证，其高质量的产品和专业的研发精神已被全球客户认可，被公认/票选为细胞信号研究的金标准、最佳抗体*、研究者的选择*、PTM（蛋白翻译后修饰）年度抗体公司*、十年抗体品牌*等（*来自CiteAb、LISA数据报告）。

CST提供最高品质的特色信号蛋白及磷酸化、甲基化、乙酰化、泛素化、SUMO化等翻译后修饰抗体，蛋白翻译后修饰筛选试剂盒及服务，还有偶联抗体，二抗，ChIP试剂盒，细胞因子，ELISA试剂盒，细胞检测试剂盒，蛋白实验配套试剂等，为您提供蛋白相关实验的一站式解决方案。

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