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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Cell Technology/Fluoro EPO/100/FLEPO100
产品编号:FLEPO100
市  场 价:¥8300.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$415.00
品      牌: Cell Technology
公      司:CellTechnology
公司分类:
Cell Technology/Fluoro EPO/100/FLEPO100
商品介绍
  • Description
  • Additional Information
  • Readable Documents
  • Assay Principle
  • Reviews

Key Benefits

  • Can monitor multiple time points to follow kinetics.
  • One-step, no wash assay.
  • Adaptable for High Throughput format.
  • Highly Sensitive.
  • Applications – Fluorescent Plate Reader.

Additional information

Kit Size

100

Eosinophil peroxidase (EPO) is the most abundant enzyme found in eosinophils. It is the major cytotoxic agent released by activated eosinophils and uses hydrogen peroxide to generate reactive oxidants from halides and pseudo halide thiocyanate [1,2]. Eosinophils peroxidase has been shown to have antimycobacterial activity [3], however it is also implicated in tissue damage that occurs in asthma and other diseases [4,5]. Currently, the function of eosinophil involvement in the immune response is being redefined. Once considered a cell involved in host protection of parasitic infection, eosinophils multiple functions as leukocytes involved in the initiation and propagation of diverse inflammatory responses are being investigated. Eosinophils are further involved as modulators of innate and adaptive immunity [6].

H2O2 + Detection reagent (non-fluorescent)+ EPO ———> fluorescent analog

Excitation: 530-571nm; Emission: 590-600nm

eosinophil_peroxidase

Figure 1. Eosinophil peroxidase was titrated in 1X reaction buffer and 50uL of each point was added in triplicate to 96 black wells. 50uL of reaction cocktail was added and the reaction was incubated in the dark at room temperature for 30 minutes.

Fluorescence was read at 530nm excitation and emission detected at 590nm.

Document Title
EPOProtocol
Fluoro EPO Datasheet
msds.fluoroEPO
Reference
Mayeno, A. N., Curran, A. J., Roberts, R. L. and Foote, C. S. (1989) Eosinophils preferentially use bromide to generate halogenating agents. J. Biol. Chem. 264, 5660±5668 .
Slungaard, A. and Mahoney, J. R. (1991) Thiocyanate is the major substrate for eosinophil peroxidase in physiologic ¯uids. J. Biol. Chem. 266, 4903±4910 (Pruitt, K. M. and Tenovuo, J. V., eds.), pp. 31±53, Marcel Dekker, New York
Human Eosinophil Peroxidase Induces Surface Alteration, killing, and lysis of Mycobacterium tuberculosis", Infection and Immunity, Feb. 2003, p. 605-613 by The American Society for Microbiology.
Bousquet, J., Chanez, P., Lacoste, J. Y., Barneon, G., Ghavanian, N., Enander, I., Venge, P., Ahlstedt, S., Simony-Lafontaine, J., Godard, P. and Michel, F. (1990) Eosinophilic in¯ammation in asthma. New Engl. J. Med. 323, 1033±1039
Parrillo, J. E., Borer, J. S., Henry, W. L., Wolff, S. M. and Fauci, A. S. (1979) The cardiovascular manifestations of the hypereosinophilic syndrome. Prospective study of 26 patients, with review of the literature. Am. J. Med. 67, 572-582
Marc E. Rothenberg and ­Simon P. Hogan.­. THE EOSINOPHIL Annual Review of Immunology, Vol. 24: 147-174 (Volume publication date April 2006.)
Mingjie Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina and Richard P. Haugland. A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases. Anal Biochem 253, 162 (1997).
J. G. Mohanty, Jonathan S. Jaffe, Edward S. Schulman and Donald G. Raible. A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative. J. Immunol Methods 202, 133 (1997).
Tatyana V. Votyakova and Ian J. Reynolds. Membrane Potential dependent and -independent production of reactive oxygen species by rat brain mitochondria. J Neurochem 79, 266 (2001).
Chun Song, Abu B. Al-Mehdi, and Aron B. Fisher. An immediate endothelial cell signaling response to lung ischemia. Am J Physiol Lung Cell Mol Physiol 281, L993 (2001).
Samantha C. Richer and W.C.L. Ford. A critical investigation of NADPH oxidase activity in human spermatozoa. Mol Hum Reprod 7, 237 (2001).
William G. Gutheil, Miglena E. Stefanova and Robert A. Nicholas. Fluorescent Coupled Enzyme Assays for Image-Alanine: Application to Penicillin-Binding Protein and Vancomycin Activity Assays. Anal Biochem 287, 196 (2000).
Dominik Peus, Remus A. Vasa, Astrid Beyerle, Alexander Meves, Carsten Krautmacher and Mark R. Pittelkow. UVB Activates ERK1/2 and p38 Signaling Pathways via Reactive Oxygen Species in Cultured Keratinocytes. J Invest Dermatol 112, 751 (1999).
Tatyana V.Votyakova ,Ian J.Reynolds. Detection of hydrogen peroxide with Amplex Red:interference by NADH and reduced glutathione auto-oxidation. Archives of Biochemistry and Biophysics, 431: 138-144 (2004).
# D. W. R. Hall, Bridget W. Logan and G. H. Parsons. Further studies on the inhibition of monoamine oxidase by M & B 9302 (clorgyline)—I .Substrate specificity in various mammalian species. Biochemical Pharma
Part#ReagentTemperature
Part# 4016Detection Reagent, 1 Vial-20C
Part# 300210X Assay Buffer, 60mL2-8C
Part# 3012Hydrogen Peroxide, 1000µL of a Stabilized Solution2-8C
Part# 6016Eosinophil Peroxidase, 1 Vial (100µL at 10Units/mL)2-8C

Please select an ACF field to output

品牌介绍

Cell Signaling Technology (CST) 是一家由科学家创立的私营家族公司,致力于提供全球最高品质的创新研究和诊断产品,加速生物学认知以及实现个体化医疗。CST坚持自主生产和严格验证,其高质量的产品和专业的研发精神已被全球客户认可,被公认/票选为细胞信号研究的金标准、最佳抗体*、研究者的选择*、PTM(蛋白翻译后修饰)年度抗体公司*、十年抗体品牌*等(*来自CiteAb、LISA数据报告)。

CST提供最高品质的特色信号蛋白及磷酸化、甲基化、乙酰化、泛素化、SUMO化等翻译后修饰抗体,蛋白翻译后修饰筛选试剂盒及服务,还有偶联抗体,二抗,ChIP试剂盒,细胞因子,ELISA试剂盒,细胞检测试剂盒,蛋白实验配套试剂等,为您提供蛋白相关实验的一站式解决方案。


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