请使用支持JavaScript的浏览器!
主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Cell Technology/Fluoro SSAO/500/SSAO100
产品编号:SSAO100
市  场 价:¥8300.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>连接酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$415.00
品      牌: Cell Technology
公      司:CellTechnology
公司分类:
Cell Technology/Fluoro SSAO/500/SSAO100
商品介绍
  • Description
  • Additional Information
  • Readable Documents
  • Assay Principle
  • Reviews

Key Benefits

  • Non Radioactive.
  • Can monitor multiple time points to follow kinetics.
  • Monitors Enzymatic Activity.
  • One-step, no wash assay.
  • Adaptable for High Throughput format.
  • Enzyme Positive Control included in Kit.
  • Applications – Fluorescent Plate Reader.

Additional information

Kit Size

500

Semicarbazide-sensitive amine oxidase (SSAO) is a common name for a widely distributed enzyme in nature. In man this enzyme is present in the vascular system and circulates in plasma. SSAO differ from the monoamine oxidases A and B in substrate and inhibitor patterns. These enzymes have been widely studied and their tissue distribution, molecular properties, substrate specificities and inhibitor sensitivities are extensively reviewed (2,3). SSAO exists in two forms: tissue bound and soluble (plasma SSAO). Tissue bound SSAO acitvity is associated with blood vessels, mainly in smooth muscle layers, however it is also associated with spleen, placenta, bone marrow, kidney, sclera, retina, endothelial cells, adipocytes, chondrocytes and fibroblasts. (4,5). Evidence suggests that Plasma SSAO originates from the cleavage of membrane-bound form. The possible sources of plasma SSAO are still unclear, but it has been suggested that it may be derived from liver, retina, placenta and bone tissue (6,7,8). SSAO’s functional role has been suggested to be involved in: apoptosis, atherogenesis, cell adhesion, leucocyte trafficking, glucose transport and local production of hydrogen peroxide. Reports of elevated levels of SSAO have been reported in congestive heart failure, diabetes mellitus, alzheimer’s disease and various other inflammatory diseases (1). Furthermore byproducts of SSAO deamination, such as formaldehyde and methylglyoxal, have been proposed to be involved in pathogenesis of cancer, aging and atherosclerosis. The Fluoro SSAO detection kit utilizes a non- fluorescent detection reagent, to detect H202 released from the conversion of Benzylamine to Benzaldehyde via SSAO. Furthermore H202 oxidizes this detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase.

Document Title
SSAOProtocol
Fluoro SSAO Datasheet
msds.fluoroSSAO
Reference
Yayin Tahiri Kasim et.al., Turk J Biochem; 29 (3);247-254 (2004); Semicarbazide-Sensitive Amine Oxidase: Biochemical and Physiological Properties
B.A.Callingham,A.Holt,J.Elliott.J.Neural Transm.(Suppl.)32 ,279 –290 (1990).
B.A.Callingham and M.A.Barrand.J.Neural Transm.(Suppl.)23 ,37 –54 (1987).
G.A.Lyles.J.Neural Transm.(Suppl.)41 ,387 –396 (1994).
K.Magyar, Z.Mészáros and P.Mátyus . Semicarbazide-sensitive amine oxidase.Its physiological significance Pure Appl.Chem.,Vol.73,No.9,pp.1393 –1400,2001.
Boomsma F.,van Dijk J.,Bhaggoe U.M.,Bouhuizen A.M.B.,van den Meiracker A.H.(2000)Variation in semicarbazide-sensitive amine oxidase activity in plasma and tissues of mammals.Comp.Biochem.
Ekblom J.,Gronval J.L.,Garpenstrand H.,Nillson S.,Oreland L.(2000)Is semicarbazide-sensitive amine oxidase in blood plasma partly derived from the skeleton?.Neurobiology,8,129-135.
McGuirl M.A., Dooley D.M.(1999)Copper-containing oxidases. Curr.Opin.Chem.Biol.,3,138-144.
Methods Enzymology 142, 617 (1997).
Holt A. ; Sharman D.F. ; Baker G.B. ; Palcic M.M. A Continuous Spectrophotometric Assay for Monoamine Oxidase and Related Enzymes in Tissue Homogenates Analytical Biochemistry, January 1997, vol. 244, no. 2, pp. 384-392(9).
Biochem Pharmacol 18, 1447 (1969).
Part#ReagentTemperature
Part # 30195X Reaction Buffer pH 7.4, 1 Bottle2-8C
Part # 6005Horseradish Peroxidase, 1 Vial2-8C
Part # 4008Detection Reagent, 1 Vial-20C (Aliquot in Single Use Vials)
Part # 7001SSAO Substrate Benzylamine, 1 Vial-20C (Aliquot in Single Use Vials)
Part # 6006SSAO Enzyme, 1 Vial-20C (Aliquot in Single Use Vials)
Part # 7003Pargyline: Monoamine Oxidase B Inhibitor(9-11), 1 Vial-20C (Aliquot in Single Use Vials)
Part # 7004Semicarbazide-Sensitive Amine Oxidase Inhibitor, 1 Vial-20C (Aliquot in Single Use Vials)

Please select an ACF field to output

品牌介绍

Cell Signaling Technology (CST) 是一家由科学家创立的私营家族公司,致力于提供全球最高品质的创新研究和诊断产品,加速生物学认知以及实现个体化医疗。CST坚持自主生产和严格验证,其高质量的产品和专业的研发精神已被全球客户认可,被公认/票选为细胞信号研究的金标准、最佳抗体*、研究者的选择*、PTM(蛋白翻译后修饰)年度抗体公司*、十年抗体品牌*等(*来自CiteAb、LISA数据报告)。

CST提供最高品质的特色信号蛋白及磷酸化、甲基化、乙酰化、泛素化、SUMO化等翻译后修饰抗体,蛋白翻译后修饰筛选试剂盒及服务,还有偶联抗体,二抗,ChIP试剂盒,细胞因子,ELISA试剂盒,细胞检测试剂盒,蛋白实验配套试剂等,为您提供蛋白相关实验的一站式解决方案。


自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔