- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Introduction
Traditionally, formaldehyde (FA) has been viewed as an environmental hazard and a toxic carcinogen for mammals. However, FA is produced in mammalian cells via enzymatic pathways and used in the “one carbon cycle” to make building blocks for life such as DNA and certain amino acids. This ‘one carbon cycle’ is fundamental to all forms of life down to the bacterial cell (1-6). FA is maintained in homeostasis in living cells, however its breakdown and over production has been implicated in the pathogenesis of various diseases such as cancer, dementia, diabetes and Alzheimer’s disease (7-11).
Cell Technology introduces two ultra-fast reversible probes, developed by Dr. Xin Li et al, PMF and PMF4 for the detection of FA in living cells or aqueous samples (12,13). These quenched fluorogenic probes when exposed to FA become fluorescent: Ex 320-420nm Em: 520-560mm. PMF4 is especially useful in in-situ visualization of FA in cellular lysosomes.
Key Benefits
- Probe PMF: In-situ monitoring of Formaldehyde (FA) in the cytoplam.
- Probe PMF4: In-situ monitoring of Formaldehyde (FA) in lysosomes.
- Study of FA levels in cellular stress, diseases such as Alzhimers and cancer.
- Reversible probe monitoring FA homeostasis in live cells and tissue sections.
- FA detection in Bacterial, fungal and plant cells.
- Environmental samples.
Additional information
| Kit Size | 0.5mg, 2.0mg |
|---|
PMF and PMF4 are quenched fluorogenic probes and when exposed to FA become fluorescent: These probes are extremely useful for continuous in-situ monitoring of FA in live cells or live tissue sections. Ex 320-420nm Em: 520-560mm. PMF4 is especially useful in in-situ visualization of FA in cellular lysosomes.
Reaction:
1. PMF/PMF4 Non-Fluorescent Dye + Formaldehyde → Fluorescent Analog
Excitation: 320-420nm and Emission at 520-560nm. Flow Cytometery /Fluorescent Microscope Ex:488 Em: 520-560nm.
Figure. Photophysical responses of PFM to FA. (A) PFM (20 μM) was treated with various amounts of FA for 5 min, and then the UV−vis spectra were recorded. (B) Fluorescent spectra of PFM (10 μM) after the treatment of various concentrations of FA for 5 min. (C) Reaction time course of PFM (10 μM) with FA (10, 100, 200, 400 μM). (D) Fluorescent responses of PFM (10 μM) toward various analytes (300 μM) after a reaction time of 30 min without (black) or with (red) the presence of FA (300 μM). The analytes tested were PFM blank (1), acetaldehyde (2), malonaldehyde (3), ascorbic acid (4), glucose (5), glucosone (6), oxalic acid (7), pyruvate (8), methylglyoxal (9), glyoxal (10), p-methoxybenzaldehyde (11), trichloroacetaldehyde (12), p-nitrobenzaldehyde (13), acetone (14), HClO (15), H2O2 (16), GSH (17). For C and D, F represents the fluorescent intensity of PFM at 500 nm after the treatment of FA for various time or after the treatment of various analytes, and F0 represents the intensity of blank PFM solution at 500 nm. All data were collected in PBS (pH 7.4, 10 mM) at ambient temperature with λex 451 nm.
| Reference |
| Yu PH, Wright S, Fan EH, Lun ZR, Gubisne-Harberle D. Physiological and pathological implications of semicarbazide-sensitive amine oxidase. Biochim Biophys Acta. 2003; 1647: 193-9. |
| O"Sullivan J, Unzeta M, Healy J, O"Sullivan MI, Davey G, Tipton KF. Semicarbazide-sensitive amine oxidases: enzymes with quite a lot to do. Neurotoxicology. 2004; 25: 303-15 |
| Lee ES, Chen H, Hardman C, Simm A, Charlton C. Excessive S-adenosyl-L-methionine-dependent methylation increases levels of methanol, formaldehyde and formic acid in rat brain striatal homogenates: possible role in S-adenosyl-L-methionine-induced Parkinson"s disease-like disorders. Life Sci. 2008; 83: 821-7. |
| Shi Y, Lan F, Matson C, Mulligan P, Whetstine JR, Cole PA, Casero RA, Shi Y. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 2004; 119: 941-53. |
| WangY, ZhangH, ChenY, SunY, YangF, YuW,LiangJ, SunL, YangX, ShiL, Li R, Li Y, Zhang Y, Li Q, Yi X, Shang Y. LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer. Cell 2009; 138: 660-72. 6. Guillermo Burgos-Barragan, Niek Wit, Johannes Meiser, Felix A. Dingler, Matthias Pietzke, Lee Mulderrig, Lucas B. Pontel, Ivan V. Rosado, Thomas F. Brewer, Rebecca L. Cordell, Paul S. Monks, Christopher J. Chang, Alexei Vazquez, Ketan J. Patel. Mammals divert endogenous genotoxic formaldehyde into one-carbon metabolism. Nature, 2017; |
| A. R. Hipkiss, Aging Dis., 2017, 8, 128–130. |
| R. Baan, Y. Grosse, K. Straif, B. Secretan, F. El Ghissassi, V. Bouvard, L. Benbrahim-Tallaa, N. Guha, C. Freeman, L. Galichet, V. Cogliano and W. H. O. I. A. f. R. o. C. M. W. Group, Lancet Oncol., 2009, 10, 1143–1144. |
| J. D. Rizak, Y. Ma and X. Hu, Curr. Alzheimer Res., 2014, 11, 461–468. 20 J. Lu, J. Miao, T. Su, Y. Liu and R. He, Biochim. Biophys. Acta, 2013, 1830, 4102–4116. |
| T. Jiang, Q. Sun and S. Chen, Prog. Neurobiol., 2016, 147, 1–19. |
| A. Rahmadi, N. Steiner and G. Munch, Clin. Chem. Lab. Med., 2011. |
| Xing-Guang Liang, Bo Chen, Ling-Xiao Shao, Juan Cheng, Ming-Zhu Huang, Yu Chen, Yong-Zhou Hu, Yi-Feng Han, Feng Han and Xin Li.A Fluorogenic Probe for Ultrafast and Reversible Detection of Formaldehyde in Neurovascular Tissues. Theranostics, 2017:7(8): 2305-2313. doi: 10.7150/thno.19554 |
| Part# | Reagent | Temperature |
| 4029 | PMF: 1 vial | -20°C |
Cell Signaling Technology (CST) 是一家由科学家创立的私营家族公司，致力于提供全球最高品质的创新研究和诊断产品，加速生物学认知以及实现个体化医疗。CST坚持自主生产和严格验证，其高质量的产品和专业的研发精神已被全球客户认可，被公认/票选为细胞信号研究的金标准、最佳抗体*、研究者的选择*、PTM（蛋白翻译后修饰）年度抗体公司*、十年抗体品牌*等（*来自CiteAb、LISA数据报告）。
CST提供最高品质的特色信号蛋白及磷酸化、甲基化、乙酰化、泛素化、SUMO化等翻译后修饰抗体，蛋白翻译后修饰筛选试剂盒及服务，还有偶联抗体，二抗，ChIP试剂盒，细胞因子，ELISA试剂盒，细胞检测试剂盒，蛋白实验配套试剂等，为您提供蛋白相关实验的一站式解决方案。
本网站将在规定时间内给予删除等相关处理。
