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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Cell Technology/Fluoro Phosphate/100/FLPHOS100
产品编号:FLPHOS100
市  场 价:¥4700.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$235.00
品      牌: Cell Technology
公      司:CellTechnology
公司分类:
Cell Technology/Fluoro Phosphate/100/FLPHOS100
商品介绍
  • Description
  • Additional Information
  • Readable Documents
  • Assay Principle
  • Reviews

Introduction

Phosphorus is essential for multiple and diverse biological functions, including cellular signal transduction, mineral meatbolism, and energy exchange. Elevated levels of serum phosphate may be associated with a higher risk of death and adverse cardiovascular outcomes in people with prior myocardial infarction, and is the subject of large amount of research.

Key Benefits

  • Quantitation of phosphate anion in blood, plasma and serum samples.
  • Sensitive – can detect upto 200nM phosphate
  • Easy to use – Fluorescence (ex/em 535/585nm or Absorbance readout @ 570nm)

Additional information

Kit Size

100

Cell Technology’s Phosphate detection kit provides a simple, one-step fluorimetric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. The Hydrogen Peroxide then reacts with the detection reagent in a 1:1 stoichiometry in presence of Horse Radish Peroxidase to produce the stable fluorescent product.

Substrate + Pi

↓ Coupled enzyme reaction

H2O2

↓ Horse Radish Peroxidase + Detection Reagent

Fluorescent Product

λmax 570nm. λ ex/em 535/585nm.

Colorimetric assay can be read on a spectrophotometer at 570nm.

Fluorescence is measured at excitation 530nm and emission 585nm.

Fig.1 Phosphate standard curve was generated using fluorimetric detection: excitation 535nm and emission 585nm. Incubation time= 60 minutes at 37oC. Standard curve range 1.5625 µM to 100µM. Graph was plotted using 4PL non-linear regression.

Fig.2 Phosphate standard curve inset: R2 =0.9835. Incubation time=60 minutes at 37oC. Standard curve range 1.5625µM to 25µM. Graph plotted with Linear regression.

Document Title
Fluoro Phosphate Protocol
Fluoro Phosphate Datasheet
msds.fluoroPhos
Reference
Mohanty, J.G. et al : A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative, J.Immunological Methods, 202, issue 2, P.133-141 (1997)
Tonelli, M. et al : Relation between serum phosphate level and cardiovascular event rate in people with coronary disease, Circulation,112, P.2627-2633 (2005)
Part#ReagentTemperature
Part # 702325X Substrate-20C
Part # 4025Detection Reagent-20C
Part # 6026Enzyme A-20C
Part # 6027Enzyme B-20C
Part # 3060Phosphate Standard2-8C
Part # 6028Horseradish Peroxidase Enzyme2-8C
Part # 30591X Reaction Buffer2-8C
Part # 3061Sample Diluent2-8C
1 Black Clear-Bottom 96-Well Plate for Fluorescent Plate Reader

Please select an ACF field to output

品牌介绍

Cell Signaling Technology (CST) 是一家由科学家创立的私营家族公司,致力于提供全球最高品质的创新研究和诊断产品,加速生物学认知以及实现个体化医疗。CST坚持自主生产和严格验证,其高质量的产品和专业的研发精神已被全球客户认可,被公认/票选为细胞信号研究的金标准、最佳抗体*、研究者的选择*、PTM(蛋白翻译后修饰)年度抗体公司*、十年抗体品牌*等(*来自CiteAb、LISA数据报告)。

CST提供最高品质的特色信号蛋白及磷酸化、甲基化、乙酰化、泛素化、SUMO化等翻译后修饰抗体,蛋白翻译后修饰筛选试剂盒及服务,还有偶联抗体,二抗,ChIP试剂盒,细胞因子,ELISA试剂盒,细胞检测试剂盒,蛋白实验配套试剂等,为您提供蛋白相关实验的一站式解决方案。


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