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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Cell Technology/SOD/100/CSOD100
产品编号:CSOD100
市  场 价:¥4900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$245.00
品      牌: Cell Technology
公      司:CellTechnology
公司分类:
Cell Technology/SOD/100/CSOD100
商品介绍
  • Description
  • Additional Information
  • Readable Documents
  • Assay Principle
  • Reviews

Superoxide dismutase (SOD) are metalloenzymes that catalyze the dismutation of superoxide radical into hydrogen peroxide (H2O2) + molecular oxygen (O2) and consequently provide an important defense mechanism against superoxide radical toxicity (1). Oxidative stress dependent upon superoxide radical can account for a number of acute and chronic disease states, which include inflammation and ischemia-reperfusion (2,3). SOD protects murine peritoneal macrophages from apoptosis induces by adriamycine (4). Furthermore, over expression of SOD in fibrosarcome cells, protects against apoptosis and promotes cell differentiation (5).

Key Benefits

  • 100% Inhibition by Super Oxide Dismutase (SOD).
  • Can detect low concentrations of SOD.
  • Highly water-soluble formazan dye.
  • Applications: Colorimetric detection.

Additional information

Kit Size

100

Cell Technology’s SOD kit utilizes a water-soluble tetrazolium salt (WST-1) that produces a highly water-soluble formazan dye upon reduction with a superoxide anion (6). The rate of the reduction with O2.- is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD, as shown in Figure 1. Therefore, the IC 50 (50% inhibition activity of SOD or SOD-like materials) can be determined by colorimetric method. Absorbance can be measured at 440nm.

Other direct and indirect methods have been developed but have issues with poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. Cytochrome C is also commonly used for SOD activity detection, however, its reactivity with superoxide is too high to determine low levels of SOD activity.

Figure 1. Inhibition Curve Prepated Using SOD from Bovine Liver.

Figure 2. SOD ASSAY Reaction

Document Title
SODProtocol
SOD Datasheet
msds.CSOD
TitleFileLinkAuthor(s)JournalYear; Edition:Pages
Photocatalytic disinfection of marine bacteria using fluorescent lighthttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V73-4TGS7BN-1&_user=10&_coverDate=12%2F31%2F2008&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1287271047&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a338bc47cd932e5bdf08ed85a516dd12T.Y Leung, C. Y Chan,C. Hu, J.C Yu and p.k. wongWater Researchvol 42 issue 19 - December 2008 pp 4827-4837
Vascular oxidative stress and nitric oxide depletion in HIV-1 transgenic rats are reversed by glutathione Restorationhttp://ajpheart.physiology.org/content/294/6/H2792.shortErik R. Kline, Dean J. Kleinhenz, Bill Liang, Sergey Dikalov, David M. Guidot, C. Michael Hart, Dean P. Jones, and Roy L. SutliffAJP - HeartJune 2008 vol. 294 no. 6, H2792-H2804
Reference
Malstrom, B., Andreasson, L., and Reinhammer, B. in The Enzymes. Byer, P., editor. XIIB, Academic Press, New York (1975).
Lontz, W., Sirsjo, J., Liu, W., Lindberg, M., Rollman, O., and G. (1976) Int. J. Cancer 17, 62–70. Torma, H. (1995) Free Radical Biol. Med. 18, 349–355.
Janero, D. R. (1995) CRC Crit. Revs. Food Sci. Nutr. 35, 65–81
Dominguez-Rodriguez, J.R. et al. (2001) Anticancer Res. 21:1869.
Zhao, Y. et al. (2001) Antioxid. Redox Signal 3:375.
# H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999).
Part#ReagentTemperature
Part # 401420X WST-1 Solution, 1 ml2-8°C
Part # 6010Xanthine Oxidase Solution (XO), 20uL2-8°C
Part # 3029Assay Buffer, 20 mL2-8°C
Part # 3030Xanthine Oxidase Dilution Buffer, 10mL. (XO Dilution Buffer)2-8°C
Part # 6011SOD Enzyme, 30uL. See vial for acivity2-8°C
Part # 900196 Well ELISA Plate, 1 plate2-8°C
Part # 9002Adhesive Plate Cover, Qty. 22-8°C

Please select an ACF field to output

品牌介绍

Cell Signaling Technology (CST) 是一家由科学家创立的私营家族公司,致力于提供全球最高品质的创新研究和诊断产品,加速生物学认知以及实现个体化医疗。CST坚持自主生产和严格验证,其高质量的产品和专业的研发精神已被全球客户认可,被公认/票选为细胞信号研究的金标准、最佳抗体*、研究者的选择*、PTM(蛋白翻译后修饰)年度抗体公司*、十年抗体品牌*等(*来自CiteAb、LISA数据报告)。

CST提供最高品质的特色信号蛋白及磷酸化、甲基化、乙酰化、泛素化、SUMO化等翻译后修饰抗体,蛋白翻译后修饰筛选试剂盒及服务,还有偶联抗体,二抗,ChIP试剂盒,细胞因子,ELISA试剂盒,细胞检测试剂盒,蛋白实验配套试剂等,为您提供蛋白相关实验的一站式解决方案。


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