Cell Technology/Fluoro H202/500/FLOH100,Cell Technology,,Cell Technology,celltechnology,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Cell Technology/Fluoro H202/500/FLOH100

产品编号：FLOH100

市场价：¥4900.00

场地：美国(厂家直采)

产品分类：分子类>二代测序>测序酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$245.00

品牌： Cell Technology

公司：CellTechnology

公司分类：

商品介绍

Description
Additional Information
Readable Documents
Assay Principle
Reviews

Key Benefits

Quick 10 minute assay.
Can monitor multiple time points to follow kinetics.
Dual mode, can detect H202 or peroxidase activity.
One-step, no wash assay.
Adaptable for High Throughput format.
Non-destructive cell based assay allows monitoring of additional parameters.
Applications – Fluorescent Plate Reader.

Additional information

Kit Size	500

The Fluoro H₂O₂ detection kit utilizes a non-fluorescent detection reagent, to detect H₂O₂. H₂O₂ oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

The detection reagent can be utilized to detect H₂O₂ release from cells or enzyme coupled reactions (1-7)

flouro4-f1

Figure 1. Hydrogen Peroxide titrated in 1X reaction Buffer and measured using the Fluoro H₂O₂ Kit.

Document Title

Fluoro H2O2Protocol

Fluoro H2O2 Datasheet

msds.fluoroh2o2

Title	File	Link	Author(s)	Journal	Year; Edition:Pages
Deficiency of the Bax gene attenuates denervation-induced apoptosis		http://www.springerlink.com/content/p1n841w124828181/	P. M. Siu and S. E. Alway	Apoptosis Journal	Vol 11/No 6 June 2006
Manganese chloride stimulates rat microglia to release hydrogen peroxide		http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCR-4P2S92J-1&_user=10&_coverDate=09%2F10%2F2007&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722767089&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7f28a0171643f2629b142edec039c748&searchtype=a	Ping Zhang, Angela Hatter and Bin Liu	Toxicology Letters	Volume 173, Issue 2, 10 September 2007, Pages 88-100 -doi:10.1016/j.toxlet.2007.06.013
Superoxide dismutase protects against apoptosis and alveolar enlargement induced by ceramide		http://ajplung.physiology.org/content/295/1/L44.short	Irina Petrache, Terry R. Medler, Amy T. Richter, Krzysztof Kamocki, Ugonma Chukwueke, Lijie Zhen, Yuan Gu, Jeremy Adamowicz, Kelly S. Schweitzer,1 Walter C. Hubbard, Evgeny V. Berdyshev, Giuseppe Lungarella, and Rubin M. Tuder	American Journal of Physiology, Lung Physiol	July 2008, Vol 295, No 1 L44-L53
Dual Role of Vitamin C Utilization in NO2-Induced Oxidative Stress in Lung Tissues of Mice		http://www.springerlink.com/content/j0870103v5820t78/	Yingyi Zhang, Chunyan Ma, Yuhuan Xiao, Chang Liu, Qiang Xu, Yan Wang, Xin Xu and Lin Hao	Bulletin of Environmental Contamination and Toxicology	Vol 84, Number 6, 662-666 - DOI: 10.1007/s00128-010-0021-1
Cigarette Smoke-Generated Reactive Oxygen Species Impair Adenosine-Mediated Wound Closure in Bronchial Epithelial Cells		http://ajrccm.atsjournals.org/cgi/content/citation/179/1_MeetingAbstracts/A2382	DS Allen-Gipson, JC Jarrell, and H Alvarez-Ramirez	American Journal of Respiratory and Critical care medicine	2009; 179: A2382
Benzyl isothiocyanate-mediated generation of reactive oxygen species causes cell cycle arrest and induces apoptosis via activation of MAPK in human pancreatic cancer cells		http://carcin.oxfordjournals.org/cgi/content/abstract/30/10/1744	Ravi P. Sahu, Ruifen Zhang, Sanjay Batra, Yan Shi and Sanjay K. Srivastava	Carcinogenesis	2009 30(10):1744-1753
Vibration Disrupts Vascular Function in a Model of Metabolic Syndrome		http://www.jstage.jst.go.jp/article/indhealth/47/5/47_533/_article	Kristine Krajnak, Stacey Waugh, Claud Johnson, Roger Miller, Meagan Kiedrowski	Industrial Health	Vol 47 (2009), No 5, pp 533-542
Inhibition of xanthine oxidase reduces oxidative stress and improves skeletal muscle function in response to electrically stimulated isometric contractions in aged mice		http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T38-52JVX0M-1&_user=10&_coverDate=04%2F07%2F2011&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722912834&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=daa9b274fb07dfe348917b389f76c96b&searchtype=a	Michael J. Ryan, Janna R. Jackson, Yanlei Hao, Stephen S. Leonard and Stephen E. Alway	Free Radical Biology and Medicine	April 2011, doi:10.1016/j.freeradbiomed.2011.04.002

Reference

Anal Biochem 253, 162 (1997)

J. Immunol Methods 202, 133 (1997)

J Neurochem 79, 266 (2001)

Am J Physiol Lung Cell Mol Physiol 281, L993 (2001)

Mol Hum Reprod 7, 237 (2001)

Willingham MC (1999) Cytochemical methods for the detection of apoptosis. J Histochem Cytochem 47:1101–1109

J Invest Dermatol 112, 751 (1999)

Part#	Reagent	Temperature
Part # 3011	5X Reaction Buffer: 20 ml buffer, pH 7.4	2-8°C
Part # 4007	Detection reagent: One vial for 500 assays.	2-8°C
Part # 3012	Hydrogen Peroxide: 200µL of a stabilized 3% solution.	2-8°C
Part # 6004	Horseradish Peroxidase: 18.9 Units of enzyme	2-8°C

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品牌介绍

Cell Signaling Technology (CST) 是一家由科学家创立的私营家族公司，致力于提供全球最高品质的创新研究和诊断产品，加速生物学认知以及实现个体化医疗。CST坚持自主生产和严格验证，其高质量的产品和专业的研发精神已被全球客户认可，被公认/票选为细胞信号研究的金标准、最佳抗体*、研究者的选择*、PTM（蛋白翻译后修饰）年度抗体公司*、十年抗体品牌*等（*来自CiteAb、LISA数据报告）。

CST提供最高品质的特色信号蛋白及磷酸化、甲基化、乙酰化、泛素化、SUMO化等翻译后修饰抗体，蛋白翻译后修饰筛选试剂盒及服务，还有偶联抗体，二抗，ChIP试剂盒，细胞因子，ELISA试剂盒，细胞检测试剂盒，蛋白实验配套试剂等，为您提供蛋白相关实验的一站式解决方案。

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